Emulsion PCR: Techniques and Applications | Springer Nature Experiments (2025)

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Abstract Figures (0) & Videos (0) Experimental Specifications Techniques Reagents Other Keywords Techniques Reagents Other Keywords Citations (9) Discover related articles Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols References Abstract Experimental Specifications Techniques Reagents Other Keywords Techniques Reagents Other Keywords Discover related articles Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols References Figures (0) & Videos (0) Citations (9) References

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Emulsion PCR: Techniques and Applications | Springer Nature Experiments (3)

Authors:

Rashmi Kanagal-Shamanna 1

Rashmi Kanagal-Shamanna 1

Show more details

Series: Methods In Molecular Biology > Book: Clinical Applications of PCR

Protocol | DOI: 10.1007/978-1-4939-3360-0_4

Affiliations:

  1. Department of Hematopathology, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA

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Citations: 9

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (6) Access enabled via: An Institution

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Abstract

Emulsion PCR (EmPCR) is a commonly employed method for template amplification in multiple NGS-based sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil

…more

Emulsion PCR (EmPCR) is a commonly employed method for template amplification in multiple NGS-based sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. We describe the methods of preparation and enrichment of template-positive Ion PGM™ Template OT2 200 Ion Sphere™ Particles (ISPs) on the Ion Personal Genome Machine® (PGM™) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouch™ System that includes the Ion OneTouch™ 2 and the Ion OneTouch™ ES instruments for template generation and enrichment of template-positive ISPs, respectively.

less

Figures (0) & Videos (0)

No figures & videos available

Experimental Specifications

Techniques

Reagents

Other Keywords

Emulsion PCR

Real-time PCR

PCR

Antibodies

Protein

Experimental Models

Kits

Please check back later as we are actively working on gathering the necessary information. Thank you for your understanding and patience.

Ion PGM™

Ion Sphere™ Particles (ISPs)

Emulsion PCR

Real-time PCR

PCR

Reagents

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (8)

Please check back later as we are actively working on gathering the necessary information. Thank you for your understanding and patience.

Other Keywords

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (9)

Ion PGM™

Ion Sphere™ Particles (ISPs)

Citations (9)

Discover related articles

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (10) Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (11)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (12) RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (13)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (14) An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (15)

Show more

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (16) Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (17)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (18) RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (19)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (20) An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (21)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (22) Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols

Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (23)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (24) Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols

Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (25)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (26) Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols

Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (27)

less

References

  1. Metzker ML (2010) Sequencing technologies – the next generation. Nat Rev Genet 11:31–46
  2. Gullapalli RR, Desai KV, Santana-Santos L et al (2012) Next generation sequencing in clinical medicine: challenges and lessons for pathology and biomedical informatics. J Pathol Inform 3:40
  3. Mamanova L, Coffey AJ, Scott CE et al (2010) Target-enrichment strategies for next-generation sequencing. Nat Methods 7:111–118

    4. Show more references

  4. Metzker ML (2010) Sequencing technologies – the next generation. Nat Rev Genet 11:31–46
  5. Gullapalli RR, Desai KV, Santana-Santos L et al (2012) Next generation sequencing in clinical medicine: challenges and lessons for pathology and biomedical informatics. J Pathol Inform 3:40
  6. Mamanova L, Coffey AJ, Scott CE et al (2010) Target-enrichment strategies for next-generation sequencing. Nat Methods 7:111–118
  7. Buermans HP, den Dunnen JT (2014) Next generation sequencing technology: advances and applications. Biochim Biophys Acta 1842(10):1932–1941
  8. Nakano M, Komatsu J, Matsuura S et al (2003) Single-molecule PCR using water-in-oil emulsion. J Biotechnol 102:117–124
  9. Dressman D, Yan H, Traverso G et al (2003) Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proc Natl Acad Sci U S A 100:8817–8822
  10. Meyerhans A, Vartanian JP, Wain-Hobson S (1990) DNA recombination during PCR. Nucleic Acids Res 18:1687–1691
  11. Williams R, Peisajovich SG, Miller OJ et al (2006) Amplification of complex gene libraries by emulsion PCR. Nat Methods 3:545–550
  12. Xuan J, Yu Y, Qing T et al (2013) Next-generation sequencing in the clinic: promises and challenges. Cancer Lett 340:284–295
  13. Yu B (2014) Setting up next-generation sequencing in the medical laboratory. Methods Mol Biol 1168:195–206

    14. less

Abstract

Emulsion PCR (EmPCR) is a commonly employed method for template amplification in multiple NGS-based sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil

…more

Emulsion PCR (EmPCR) is a commonly employed method for template amplification in multiple NGS-based sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. We describe the methods of preparation and enrichment of template-positive Ion PGM™ Template OT2 200 Ion Sphere™ Particles (ISPs) on the Ion Personal Genome Machine® (PGM™) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouch™ System that includes the Ion OneTouch™ 2 and the Ion OneTouch™ ES instruments for template generation and enrichment of template-positive ISPs, respectively.

less

Experimental Specifications

Techniques

Reagents

Other Keywords

Emulsion PCR

Real-time PCR

PCR

Antibodies

Protein

Experimental Models

Kits

Please check back later as we are actively working on gathering the necessary information. Thank you for your understanding and patience.

Ion PGM™

Ion Sphere™ Particles (ISPs)

Techniques

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (28)

Emulsion PCR

Real-time PCR

PCR

Reagents

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (29)

Please check back later as we are actively working on gathering the necessary information. Thank you for your understanding and patience.

Other Keywords

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (30)

Ion PGM™

Ion Sphere™ Particles (ISPs)

Discover related articles

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (31) Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (32)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (33) RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (34)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (35) An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (36)

Show more

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (37) Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Microfluidics-Based PCR for Fusion Transcript Detection 2016, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (38)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (39) RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

RNA-Seq analysis to capture the transcriptome landscape of a single cell 2010, Nature Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (40)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (41) An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

An Emulsion Polymerase Chain Reaction–Based Method for Molecular Haplotyping 2008, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (42)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (43) Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols

Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding 2023, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (44)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (45) Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols

Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX 2023, Springer Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (46)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (47) Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols

Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M 2020, Nature Protocols

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (48)

less

Advertisement

References

  1. Metzker ML (2010) Sequencing technologies – the next generation. Nat Rev Genet 11:31–46
  2. Gullapalli RR, Desai KV, Santana-Santos L et al (2012) Next generation sequencing in clinical medicine: challenges and lessons for pathology and biomedical informatics. J Pathol Inform 3:40
  3. Mamanova L, Coffey AJ, Scott CE et al (2010) Target-enrichment strategies for next-generation sequencing. Nat Methods 7:111–118

    4. Show more references

  4. Metzker ML (2010) Sequencing technologies – the next generation. Nat Rev Genet 11:31–46
  5. Gullapalli RR, Desai KV, Santana-Santos L et al (2012) Next generation sequencing in clinical medicine: challenges and lessons for pathology and biomedical informatics. J Pathol Inform 3:40
  6. Mamanova L, Coffey AJ, Scott CE et al (2010) Target-enrichment strategies for next-generation sequencing. Nat Methods 7:111–118
  7. Buermans HP, den Dunnen JT (2014) Next generation sequencing technology: advances and applications. Biochim Biophys Acta 1842(10):1932–1941
  8. Nakano M, Komatsu J, Matsuura S et al (2003) Single-molecule PCR using water-in-oil emulsion. J Biotechnol 102:117–124
  9. Dressman D, Yan H, Traverso G et al (2003) Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proc Natl Acad Sci U S A 100:8817–8822
  10. Meyerhans A, Vartanian JP, Wain-Hobson S (1990) DNA recombination during PCR. Nucleic Acids Res 18:1687–1691
  11. Williams R, Peisajovich SG, Miller OJ et al (2006) Amplification of complex gene libraries by emulsion PCR. Nat Methods 3:545–550
  12. Xuan J, Yu Y, Qing T et al (2013) Next-generation sequencing in the clinic: promises and challenges. Cancer Lett 340:284–295
  13. Yu B (2014) Setting up next-generation sequencing in the medical laboratory. Methods Mol Biol 1168:195–206

    14. less

Figures (0) & Videos (0)

No figures & videos available

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Citations (9)

Emulsion PCR: Techniques and Applications | Springer Nature Experiments (2025)

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